Figure 1.
![Figure 1. TIN2 and SA1 are required for telomere and arm cohesion, whereas SA2 is required for centromere cohesion. FISH analysis of siRNA-treated HeLaI.2.11 mitotic cells with chromosome-specific fluorescently labeled probes: (A–D) telomere 16pter (green), (E–H) centromere 6cen (red), and (I–L) arm 20p12 (white). The cen locus is trisomic. DNA was stained with DAPI (blue). Bar, 5 μm. Histograms (based on 100 measurements from two independent experiments [see Table S1]) showing the distance between FISH signals are on the right. Average (Avg) distance with SEs is indicated. (M) Graphical representation of the average distance from two independent experiments.](https://cdn.rupress.org/rup/content_public/journal/jcb/187/2/10.1083_jcb.200903096/6/jcb_200903096_rgb_fig1.jpeg?Expires=1773462207&Signature=bzPGd7KrTNdyICjeIl0JWyrpakpsgZEwEKAOKuyJ7g2pHW-LsNXIcDo-EDlP0itiVJ6e8pt19fJuL0HvNf8hbGTkhPpemqKTHZHiycG6ABDRfnkGEvjNjgjtsjfgOz3DIuBiMkLVHmkIxqvSqZvZhvQfBKc5P2TyenkEcts4c7NSWLd-UTMb02EGdbuheK9KS1y8CJCnKob-xetXNY~oUdlB3cf-YTPUZuIisVrL9ztmz18tpj~e378U7e7YpjeX7Tfpz-ozMwKHgcrUzOq0lhfZFkp37CAHGITtFjblX-lIq9nbDErM8Lh-1T95NXMaYRe4k3r25jm34BFV8Pm74Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
TIN2 and SA1 are required for telomere and arm cohesion, whereas SA2 is required for centromere cohesion. FISH analysis of siRNA-treated HeLaI.2.11 mitotic cells with chromosome-specific fluorescently labeled probes: (A–D) telomere 16pter (green), (E–H) centromere 6cen (red), and (I–L) arm 20p12 (white). The cen locus is trisomic. DNA was stained with DAPI (blue). Bar, 5 μm. Histograms (based on 100 measurements from two independent experiments [see Table S1]) showing the distance between FISH signals are on the right. Average (Avg) distance with SEs is indicated. (M) Graphical representation of the average distance from two independent experiments.
