Wnd/JNK signaling is not required for stability. Retraction frequency is quantified for multiple genetic manipulations of MAPK signaling. Quantification of retraction frequency is measured as the average percentage of NMJs with >1 bouton retracted. puc Oe = elav^C155-GAL4/+; UAS-puc (n = 97 NMJs/10 animals). wnd²/wnd³ (n = 105 NMJs/11 animals). JNK^DN = elav^C155-GAL4/+; UAS-JNK^DN (n = 108 NMJs/11 animals). kay¹/+ (n = 118 NMJs/12 animals). kay²(L2) (n = 72 NMJs/9 animals). Error bars represent SEM. Neither one-way ANOVA nor Student's t test determined any significant differences in mean retraction frequency between genotypes.