Figure 2. Pds5 mediates chromosome morphological changes during meiosis. (A) Chromosome morphology at meiotic prophase I. Yeast cells were induced to enter synchronous meiosis for 5 (wild type [WT]) and 7 h (PCLB2PDS5), and nuclear spreads were prepared for immunofluorescence microscopy. Rec8-3HA and Sgo1-9MYC were detected with anti-HA (12CA5) and anti-MYC (9E10) antibodies, respectively. The chromosome number in PCLB2PDS5 cells appears to be twice that in wild-type cells. Red, DNA stained by DAPI; green, Rec8-3HA. (B) ChIP assay of Rec8 association with the chromosome. ChIP was performed as in Fig. 1 C. The locations of CEN3 and CARC3 are depicted on chromosome III. S. cerevisiae genome database coordinates are shown on the x axis. Error bars indicate SD. (C) Chromosome axis revealed by Red1 staining. Meiotic nuclear spreads were prepared as in A. Red1 (green) and Rec8-3HA (red) were detected with anti-Red1 and anti-HA antibodies, respectively.
Figure 2.

Pds5 mediates chromosome morphological changes during meiosis. (A) Chromosome morphology at meiotic prophase I. Yeast cells were induced to enter synchronous meiosis for 5 (wild type [WT]) and 7 h (PCLB2PDS5), and nuclear spreads were prepared for immunofluorescence microscopy. Rec8-3HA and Sgo1-9MYC were detected with anti-HA (12CA5) and anti-MYC (9E10) antibodies, respectively. The chromosome number in PCLB2PDS5 cells appears to be twice that in wild-type cells. Red, DNA stained by DAPI; green, Rec8-3HA. (B) ChIP assay of Rec8 association with the chromosome. ChIP was performed as in Fig. 1 C. The locations of CEN3 and CARC3 are depicted on chromosome III. S. cerevisiae genome database coordinates are shown on the x axis. Error bars indicate SD. (C) Chromosome axis revealed by Red1 staining. Meiotic nuclear spreads were prepared as in A. Red1 (green) and Rec8-3HA (red) were detected with anti-Red1 and anti-HA antibodies, respectively.

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