FOCAL POINT Top: Asymmetry might set the cytokinesis clock, Dannel McCollum (left) and Juan Carlos García-Cortés (right) determined. The cytokinesis-triggering septum initiation network (indicated by a bright dot on the spindle pole body) turns on only in one side of these yeast cells. Bottom: Eric Griffis (left) and Ron Vale (right), together with James Spudich, reveal that microtubules and the motor protein Kinesin-6 help dictate where cytokinesis occurs. Here, Kinesin-6 (green) has migrated to the equator of a mitotic cell.