Figure 5. Expression of Rac1 in the nucleus peaks in G2. (A) PAE cells stably expressing GFP-Rac1 at levels below endogenous were synchronized in G1/S by serum starvation followed by hydroxyurea and then released. The percentage of cells with nuclear Rac1 was determined hourly and plotted as mean ± SEM (n = 4). (B) Aliquots of the cells analyzed in A were scraped from plates at the indicated times and analyzed for stage of the cell cycle by propidium iodide and cytofluorimetry. (C) Unsynchronized COS-1 cells were transfected with GFP-Rac1 and, after 16 h, fixed and stained for cyclin A. Although the cell expressing GFP-Rac1 in the nucleus (arrows) stained for cyclin A, a marker of G2/M, those excluding the protein from the nucleus (arrowheads) did not. This correlation held for 93% of transfected cells examined (>100). Bars, 10 μm.
Figure 5.

Expression of Rac1 in the nucleus peaks in G2. (A) PAE cells stably expressing GFP-Rac1 at levels below endogenous were synchronized in G1/S by serum starvation followed by hydroxyurea and then released. The percentage of cells with nuclear Rac1 was determined hourly and plotted as mean ± SEM (n = 4). (B) Aliquots of the cells analyzed in A were scraped from plates at the indicated times and analyzed for stage of the cell cycle by propidium iodide and cytofluorimetry. (C) Unsynchronized COS-1 cells were transfected with GFP-Rac1 and, after 16 h, fixed and stained for cyclin A. Although the cell expressing GFP-Rac1 in the nucleus (arrows) stained for cyclin A, a marker of G2/M, those excluding the protein from the nucleus (arrowheads) did not. This correlation held for 93% of transfected cells examined (>100). Bars, 10 μm.

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