Figure 4. The effect of cell cycle... | Rockefeller University Press

$Figure 4. The effect of cell cycle blockade on the nuclear expression of GFP-Rac1 and endogenous Rac1. (A) PAE cells stably expressing GFP-Rac1 at levels below endogenous Rac1 (Fig. S1, available) were scored for the percentage of cells showing strong nuclear fluorescence before and 16 h after the addition of increasing amounts of the indicated compounds. Representative cells at the indicated dose are shown with the percentage of cells showing each phenotype indicated (left), and cumulative dose-response data are shown on the right (mean ± SEM; n = 3). Bars, 10 μm. (B) Endogenous Rac1, Ras, and RCC1 were measured in the nuclear fractions as described in Fig. 2 before and after the addition of 50 μM apigenin for 24 h (mean ± SEM; n = 4).$

Figure 4.

The effect of cell cycle blockade on the nuclear expression of GFP-Rac1 and endogenous Rac1. (A) PAE cells stably expressing GFP-Rac1 at levels below endogenous Rac1 (Fig. S1) were scored for the percentage of cells showing strong nuclear fluorescence before and 16 h after the addition of increasing amounts of the indicated compounds. Representative cells at the indicated dose are shown with the percentage of cells showing each phenotype indicated (left), and cumulative dose-response data are shown on the right (mean ± SEM; n = 3). Bars, 10 μm. (B) Endogenous Rac1, Ras, and RCC1 were measured in the nuclear fractions as described in Fig. 2 before and after the addition of 50 μM apigenin for 24 h (mean ± SEM; n = 4).

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