![Figure 2. Endogenous Rac1 is in the nucleus. COS-1 cells were separated into nuclear and nonnuclear fractions as described in Materials and methods. Equal cell equivalents of each fraction were analyzed by SDS-PAGE and immunoblots (inset) for the indicated proteins. Immunoprecipitated proteins were detected and quantified with [125I]protein A and phosphorimaging, and the percentage of total protein in the nuclear fraction was calculated (mean ± SEM; n = 3).](https://cdn.rupress.org/rup/content_public/journal/jcb/181/3/10.1083_jcb.200801047/7/jcb_200801047_gs_fig2.jpeg?Expires=1767657463&Signature=ryOAvKtxFg7KxPzWqTg55-EOgqMa~XuiU8vpfyezlf7g~kN4RSftt0Tdx6QsXy9DXoleB8wDvcrGOM466K1LroqFHx9BFOU--KCOC7zUpZ2g9cUGwcaQoY15CizgYpzUjXqqL5leOLl9b~iZkerYVpqfAejwmHye4Db-Ji7JoAB6QFlPifFsgGUy32bil6W43KiDjvIOlT94DL2Ekvrz0UR5RpcAVRrDaxrzk49~UxuD8TV0OxTG-L5hrsbi5MImNtkdh7uAcN85fyvcTRL68msrVglN~jvzK0jm7qYUMKTIqByTCgq9EINAnVUImWd7Aftt7l~9XfY5ykXhRZHmFQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 2.
Endogenous Rac1 is in the nucleus. COS-1 cells were separated into nuclear and nonnuclear fractions as described in Materials and methods. Equal cell equivalents of each fraction were analyzed by SDS-PAGE and immunoblots (inset) for the indicated proteins. Immunoprecipitated proteins were detected and quantified with [125I]protein A and phosphorimaging, and the percentage of total protein in the nuclear fraction was calculated (mean ± SEM; n = 3).
