Figure 1. The Rac1 NLS consists of the polybasic region in the C terminus, is strengthened by a flanking triproline motif, and is inhibited by geranylgeranylation. (A) Description of constructs used. (B) Representative distribution patters of the GFP-tagged constructs in live COS-1 and PAE cells with the percentage of cells showing the represented phenotype indicated. (C) Percentage of cells showing nuclear fluorescence greater than cytoplasmic fluorescence for the indicated constructs in MDCK, PAE, and COS-1 cells (mean ± SEM; n ≥ 4). (D) Distribution of fluorescence in COS-1 cells expressing GFP-Rac1 or GFP-Rac1/Ktail with or without treatment with 10 μM compactin. Only the nucleus is visible in cells expressing GFP-Rac1 treated with compactin. Bars, 10 μm.
Figure 1.

The Rac1 NLS consists of the polybasic region in the C terminus, is strengthened by a flanking triproline motif, and is inhibited by geranylgeranylation. (A) Description of constructs used. (B) Representative distribution patters of the GFP-tagged constructs in live COS-1 and PAE cells with the percentage of cells showing the represented phenotype indicated. (C) Percentage of cells showing nuclear fluorescence greater than cytoplasmic fluorescence for the indicated constructs in MDCK, PAE, and COS-1 cells (mean ± SEM; n ≥ 4). (D) Distribution of fluorescence in COS-1 cells expressing GFP-Rac1 or GFP-Rac1/Ktail with or without treatment with 10 μM compactin. Only the nucleus is visible in cells expressing GFP-Rac1 treated with compactin. Bars, 10 μm.

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