The JNK–c-Jun pathway does not suppress Oct-6. (A) Western blot of Schwann cells infected with adenoviruses expressing control LacZ or activated MKK7 to activate JNK. Note comparable induction of Oct-6 (by 1 mM db-cAMP for 24 h) in control cells and MKK7 cells. (B–E) Teased E17 sciatic nerve stained with HOECHST dye showing that Oct-6 and phospho–c-Jun are coexpressed in immature Schwann cells. (B and C) Double immunolabeling for phospho–c-Jun (P-cJun; red) and Oct-6 (green) in C shows coexpression in Schwann cell nuclei. (D and E) Double immunolabeling for phospho–c-Jun (red) and Krox-20 (green) in E shows phospho–c-Jun alone. Bar, 15 μm.