Persistent expression of c-Jun inhibits Krox-20–induced myelin protein expression. (A and B) Cotransfection of Krox-20/GFP with Jun(Asp) or with Jun(Ala) inhibits Krox-20–mediated induction of periaxin and P0. K20/EV represents cells cotransfected with Krox-20 and control vector. (C–F) P0 in situ experiment showing that cotransfection of Krox-20/GFP with Jun(Ala) inhibits Krox-20–mediated induction of P0 mRNA. C and D are controls, and the arrows show a cell coexpressing Krox-20 and a control vector where Krox-20 has induced P0 mRNA. Arrows in E and F show a cell coexpressing Krox-20 and Jun(Ala) where Jun(Ala) has inhibited Krox-induced P0 expression. Bar, 15 μm. (G) Percentages of GFP-positive cells that also express P0 mRNA in cells cotransfected with the constructs indicated. Error bars show one standard deviation of the mean.
