Figure 2. Dnβ1 mice express the dominant-negative β1 integrin in white matter tracts. (a) RT-PCR analysis from cortex of 3-mo-old wild-type (Wt) and transgenic mice (dnβ1) reveals RNA expression of β1 integrin in both Wt and transgenic mice but dnβ1 integrin (IL2Rα) only in the mutant. (b) Anti–human IL2Rα immunolabeling of optic nerve transverse sections (bar: 33 µm), cerebellar sagittal sections (bar: 33 µm), and corpus callosum coronal sections (bar: 66 µm) from P18 wild-type and dnβ1 mice shows that the dominant-negative integrin protein is expressed in the myelinating tracts of mutant mice. (c) Dnβ1 expression is nervous system specific as confirmed by an RT-PCR analysis on various tissues from P22 mutant mice. IL2Rα primers were used for detection of the dnβ1 expression (top). Actin primers were used as control (bottom).
Figure 2.

Dnβ1 mice express the dominant-negative β1 integrin in white matter tracts. (a) RT-PCR analysis from cortex of 3-mo-old wild-type (Wt) and transgenic mice (dnβ1) reveals RNA expression of β1 integrin in both Wt and transgenic mice but dnβ1 integrin (IL2Rα) only in the mutant. (b) Anti–human IL2Rα immunolabeling of optic nerve transverse sections (bar: 33 µm), cerebellar sagittal sections (bar: 33 µm), and corpus callosum coronal sections (bar: 66 µm) from P18 wild-type and dnβ1 mice shows that the dominant-negative integrin protein is expressed in the myelinating tracts of mutant mice. (c) Dnβ1 expression is nervous system specific as confirmed by an RT-PCR analysis on various tissues from P22 mutant mice. IL2Rα primers were used for detection of the dnβ1 expression (top). Actin primers were used as control (bottom).

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