DHHC2 is essential for activity-sensitive PSD-95 palmitoylation. (A and B) DHHC2 but not DHHC3 mediates palmitoylation of PSD-95 upon activity blockade. Knockdown of DHHC2 but not DHHC3 inhibited Kyn-induced PSD-95–GFP recruitment at the synaptic membrane. miDHHC2-resistant DHHC2 (WT) but not PAT-inactive DHHC2 (CS) rescued Kyn-induced PSD-95 accumulation. (A) TIRFM intensity of representative five punctae from a neuron was plotted with time. (B) n = 3 each; ***, P < 0.001. Error bars indicate SD. Bar, 5 µm.