Figure 4. DHHC2 and -3 are differently involved in PSD-95 trafficking. (A and B) In the DHHC2 or -3 knocked down neurons (labeled with mCherry), the number of native PSD-95 puncta (green) was significantly decreased. n = 5 neurons; ***, P < 0.001. (C and D) Knockdown of DHHC2 but not DHHC3 prevented TTX- or Kyn-induced augmentation of endogenous PSD-95 accumulation. Dashed lines (100%) indicate the normalized control level. ***, P < 0.001. (C) Analyzed by confocal laser-scanning microscopy (CLSM). n = 10–15 neurons. (D) Analyzed by TIRFM. n = 5 neurons. miLacZ is a control miRNA targeting LacZ (β-galactosidase). (B–D) Error bars indicate SD. Bar, 5 µm.
Figure 4.

DHHC2 and -3 are differently involved in PSD-95 trafficking. (A and B) In the DHHC2 or -3 knocked down neurons (labeled with mCherry), the number of native PSD-95 puncta (green) was significantly decreased. n = 5 neurons; ***, P < 0.001. (C and D) Knockdown of DHHC2 but not DHHC3 prevented TTX- or Kyn-induced augmentation of endogenous PSD-95 accumulation. Dashed lines (100%) indicate the normalized control level. ***, P < 0.001. (C) Analyzed by confocal laser-scanning microscopy (CLSM). n = 10–15 neurons. (D) Analyzed by TIRFM. n = 5 neurons. miLacZ is a control miRNA targeting LacZ (β-galactosidase). (B–D) Error bars indicate SD. Bar, 5 µm.

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