Membrane insertion of the GRASP65 N terminus is required. Schematic diagram of the constructs (A). HeLa cells expressing G65^G2A-GFP-ActA (B–D and K), T20-G65-GFP-ActA (E–G and L), or T20-GFP-ActA (H–J and M) were BFA treated and analyzed using Mitotracker (red), GFP fluorescence (green), and radial profile plots. Bar, 10 µm.