Figure 5. Endogenous GRASP65 is... | Rockefeller University Press

Figure 5.

$Figure 5. Endogenous GRASP65 is recruited to mitochondria by GM130. HeLa cells expressing T20-GFP (A–D), T20-GFP-GM130Cterm (E–H), or T20-GFP-GM130CΔ10 (J–M) were BFA treated and processed to reveal GFP fluorescence, GRASP65 staining, merged images and, from single optical sections, representations of the colocalized pixels. Bar, 10 µm. GRASP65 recruitment (N) was assayed by determining the fraction of total GFP-positive pixels in single optical sections (chosen to maximize mitochondrial representation) that colocalized with GRASP65 staining (n = 3, ±SEM, >15 cells/experiment; *, P < 0.0001).$

Endogenous GRASP65 is recruited to mitochondria by GM130. HeLa cells expressing T20-GFP (A–D), T20-GFP-GM130^Cterm (E–H), or T20-GFP-GM130^CΔ10 (J–M) were BFA treated and processed to reveal GFP fluorescence, GRASP65 staining, merged images and, from single optical sections, representations of the colocalized pixels. Bar, 10 µm. GRASP65 recruitment (N) was assayed by determining the fraction of total GFP-positive pixels in single optical sections (chosen to maximize mitochondrial representation) that colocalized with GRASP65 staining (n = 3, ±SEM, >15 cells/experiment; *, P < 0.0001).

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