Figure 2.
Figure 2. Mitochondria are tethered and not fused into syncytia. GFP-ActA (A and B) or G65-GFP-ActA (C and D) transfected HeLa cells were BFA treated, processed for electron microscopy, and shown at 2 magnifications. GFP-ActA (E–H) or G65-GFP-ActA (I–L) transfected cells were imaged live using a scanning laser microscope. A region of interest (marked in figure) was selected and bleached and recovery was monitored in subsequent frames at 2-s intervals. Bar, 2 µm. Mouse embryonic fibroblasts lacking mitofusin-1/2 and expressing the matrix marker COX-IV-DsRed were transfected with GFP-ActA (M–O) or G65-GFP-ActA (P–R), BFA-treated, and processed to reveal mitochondria (red), and the expressed proteins (green). Bar = 10 µm. An enlarged view of a single optical section is also shown (S–U). Bar = 1 µm.

Mitochondria are tethered and not fused into syncytia. GFP-ActA (A and B) or G65-GFP-ActA (C and D) transfected HeLa cells were BFA treated, processed for electron microscopy, and shown at 2 magnifications. GFP-ActA (E–H) or G65-GFP-ActA (I–L) transfected cells were imaged live using a scanning laser microscope. A region of interest (marked in figure) was selected and bleached and recovery was monitored in subsequent frames at 2-s intervals. Bar, 2 µm. Mouse embryonic fibroblasts lacking mitofusin-1/2 and expressing the matrix marker COX-IV-DsRed were transfected with GFP-ActA (M–O) or G65-GFP-ActA (P–R), BFA-treated, and processed to reveal mitochondria (red), and the expressed proteins (green). Bar = 10 µm. An enlarged view of a single optical section is also shown (S–U). Bar = 1 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal