FAK modulates the localization of pY31-paxillin, pY410 p130Cas, and Tks5 in MTLn3 cells. (A–F) MTLn3 cells transiently transfected with control siRNA (Ctlsi) or FAK siRNA (FAKsi) were cultured on fibronectin-gelatin coverslips and stained with anti-pY31 paxillin (A and B), anti-pY410 p130Cas (C and D), or anti-Tks5/FISH antibody (E and F; red). Cells were costained with the invadopodia marker cortactin (A, C, and E; green) or the focal adhesion marker vinculin (B, D, and F; green). Regions outlined by boxes correspond to magnified images of pY31-paxillin, pY410 p130Cas, or Tks5 localization at focal adhesions and/or invadopodia shown in insets. Bars, 10 µm.