Figure 5. FAK differentially regulates tyrosine phosphorylation at focal adhesions and invadopodia. (A) MTLn3 (parental) cells or v-Src–transformed MTLn3 cells transiently transfected with control siRNA (Ctlsi) or FAK siRNA (FAKsi) and cotransfected with GFP-paxillin were cultured on fibronectin-gelatin coverslips and stained with anti-p34Arc (Arp2/3 subunit) antibody and antiphosphotyrosine antibody. Two sets of overlays are depicted to illustrate localization of GFP-paxillin or Arp2/3 (green) with phosphotyrosine (pY) staining (red). (B) MTLn3 cells transiently transfected with control siRNA or FAK siRNA and cotransfected with YFP-dSH2 were cultured on fibronectin-gelatin coverslips and stained with anti-p34Arc antibody (red) and antiphosphotyrosine antibody (blue). Regions outlined by boxes indicate images of invadopodia and focal adhesions. Arrows indicate representative invadopodia, and arrowheads indicate representative focal adhesions. Bars, 10 µm.
Figure 5.

FAK differentially regulates tyrosine phosphorylation at focal adhesions and invadopodia. (A) MTLn3 (parental) cells or v-Src–transformed MTLn3 cells transiently transfected with control siRNA (Ctlsi) or FAK siRNA (FAKsi) and cotransfected with GFP-paxillin were cultured on fibronectin-gelatin coverslips and stained with anti-p34Arc (Arp2/3 subunit) antibody and antiphosphotyrosine antibody. Two sets of overlays are depicted to illustrate localization of GFP-paxillin or Arp2/3 (green) with phosphotyrosine (pY) staining (red). (B) MTLn3 cells transiently transfected with control siRNA or FAK siRNA and cotransfected with YFP-dSH2 were cultured on fibronectin-gelatin coverslips and stained with anti-p34Arc antibody (red) and antiphosphotyrosine antibody (blue). Regions outlined by boxes indicate images of invadopodia and focal adhesions. Arrows indicate representative invadopodia, and arrowheads indicate representative focal adhesions. Bars, 10 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal