Figure 2. Immunodetection of CCBs in WT and mutant cells. ccb strains: nuclear mutants affecting specifically binding of ci-heme to cyt b6. petB-C35V strain: site-directed mutant of the chloroplast petB gene encoding cyt b6 with a substitution of Cys35 preventing thioether bonding of ci-heme to cyt b6. ΔpetB strain: petB deletion strain. Whole cell proteins were separated by SDS-PAGE and analyzed by immunoblotting. A subunit of mitochondrial ATP synthase was immunodetected as a loading control (β).
Figure 2.

Immunodetection of CCBs in WT and mutant cells. ccb strains: nuclear mutants affecting specifically binding of ci-heme to cyt b6. petB-C35V strain: site-directed mutant of the chloroplast petB gene encoding cyt b6 with a substitution of Cys35 preventing thioether bonding of ci-heme to cyt b6. ΔpetB strain: petB deletion strain. Whole cell proteins were separated by SDS-PAGE and analyzed by immunoblotting. A subunit of mitochondrial ATP synthase was immunodetected as a loading control (β).

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