Figure 6. VE-PTP regulates Tie-2 phosphorylation via its active phosphatase. (A) HUVECs either untransfected, or expressing GFP or expressing mouse Flag-VE-PTP (as indicated above) were either untreated or treated with polyclonal antibodies against mouse VE-PTP or preimmune antibodies (as indicated above) for 1 h and subsequently immunoprecipitated for Tie-2, followed by immunoblotting with anti-phosphotyrosine antibodies (pTyr) and antibodies against Tie-2. Aliquots of cell lysates with identical protein content were directly immunoblotted for Flag-VE-PTP and hVE-PTP (bottom). Quantified signal intensities are indicated. (B) As in A with mouse Flag-VE-PTP being replaced by the corresponding inactive phosphatase mutant Flag-VE-PTP-C/S. White lines indicate that intervening lanes have been spliced out.
Figure 6.

VE-PTP regulates Tie-2 phosphorylation via its active phosphatase. (A) HUVECs either untransfected, or expressing GFP or expressing mouse Flag-VE-PTP (as indicated above) were either untreated or treated with polyclonal antibodies against mouse VE-PTP or preimmune antibodies (as indicated above) for 1 h and subsequently immunoprecipitated for Tie-2, followed by immunoblotting with anti-phosphotyrosine antibodies (pTyr) and antibodies against Tie-2. Aliquots of cell lysates with identical protein content were directly immunoblotted for Flag-VE-PTP and hVE-PTP (bottom). Quantified signal intensities are indicated. (B) As in A with mouse Flag-VE-PTP being replaced by the corresponding inactive phosphatase mutant Flag-VE-PTP-C/S. White lines indicate that intervening lanes have been spliced out.

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