Figure 4.
Figure 4. The GAT domain of the Gga proteins is required for a postinternalization event during Gap1 endocytosis. (A and B) Wild-type (wt), ypt6Δ, gga1Δ gga2Δ, or ypt6Δ gga1Δ gga2Δ cells were transformed with an empty centromeric vector or with a derived plasmid carrying the GGA2 or gga2ΔGAT gene. (A) Gap1 activity was measured before and at different times after the addition of ammonium. Error bars represent standard deviation. (B) Gap1 stability was analyzed in total protein extracts prepared from the same cells as in A collected before and 60 min after the addition of ammonium.

The GAT domain of the Gga proteins is required for a postinternalization event during Gap1 endocytosis. (A and B) Wild-type (wt), ypt6Δ, gga1Δ gga2Δ, or ypt6Δ gga1Δ gga2Δ cells were transformed with an empty centromeric vector or with a derived plasmid carrying the GGA2 or gga2ΔGAT gene. (A) Gap1 activity was measured before and at different times after the addition of ammonium. Error bars represent standard deviation. (B) Gap1 stability was analyzed in total protein extracts prepared from the same cells as in A collected before and 60 min after the addition of ammonium.

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