Nav1.5 expression is reduced in single rat myocytes with reduced ankyrin-G expression. (A–D) Control myocytes (uninfected; A) and cardiomyocytes transduced with control virus (YFP alone; B), rat-specific ankyrin-G shRNA virus (C), or human-specific ankyrin-G shRNA virus (D) were immunolabeled with ankyrin-G and Nav1.5-specific antibodies and imaged using identical confocal settings. Viral transduction was assessed by the presence of YFP fluorescence (pseudocolored in blue in B–D for clarity). Note that only myocytes infected with rat-specific ankyrin-G shRNA virus displayed reduced ankyrin-G expression. These myocytes consistently displayed a decreased expression of Nav1.5. In fact, Nav1.5 expression in myocytes with reduced ankyrin-G expression (arrowheads in C) was limited to the perinuclear region (arrows in C). Bars, 10 μm.
