Nav1.5 expression is reduced in rat myocytes with reduced ankyrin-G expression. Neonatal cardiomyocytes were transduced with rat-specific ankyrin-G shRNA virus. After 22 h, myocytes were collected, and whole cell lysates were generated. Equal protein concentrations were analyzed by immunoblot using affinity-purified ankyrin-G Ig and Nav1.5-specific antibody. In parallel, blots were probed with an unrelated Ig to ensure equal protein loading (NHERF1). Note that loss of 190-kD ankyrin-G expression in myocytes transduced with rat-specific ankyrin-G shRNA was paralleled by a significant reduction in Nav1.5 expression. Reduced ankyrin-G expression did not affect the expression of ankyrin-B, connexin43, or Cav1.2. Molecular masses are expressed in kilodaltons.
