Effect of EIPA in wound-healing assays on interphase NIH3T3 fibroblasts. (A) Trajectories (normalized to common starting points) of interphase NIH3T3 cells in the absence or presence of 10 µM EIPA and 50 ng/ml PDGF-AA, as indicated. The radii of the red circles illustrate the mean translocation of the cells within a 5-h time period. (B) Translocation (shown in micrometers and calculated as the distance between the position of the cell center at the beginning and at the end of the experiment) of interphase and growth-arrested NIH3T3 cells with or without EIPA, as indicated. (A and B) Data are individual trajectories (A) or means ± SEM (B) of 17–26 cells in three to four independent experiments. Data were analyzed using parametric or nonparametric ANOVA, and the level of significance is shown (*, P < 0.05). (C) Velocity of migration perpendicular to the wound in interphase NIH3T3 cells in the absence (open circles) or presence (closed circles) of 10 µM EIPA obtained as described in the legend for Fig. 3. Data shown are means ± SEM of 17–26 cells in three to four independent experiments. The velocity in the presence of EIPA is significantly different from that in the absence of EIPA (P < 0.0001). Ctrl., control.