Rescue of processive MT growth by different EB3 fusions and EB2-EB3 chimeras in EB1/EB3-delpeted cells. The proportion of MT tracks originating from the centrosome, with the length exceeding 7.5 µm, schematic representations of the constructs, amino acid positions in EB3 and EB2, and the numbers of tracks and cells analyzed for each construct are indicated. Share of long MT growth episodes in control cells, EB1/EB3-depleted cells, and cells expressing HA-EB1-ΔAc obtained using GFP-CLIP-170 or mCherry–α-tubulin (after photobleaching) is shown for comparison. Cells with approximately the same radius were selected for quantification. Error bars indicate SD determined based on 2–4 independent experiments.