Figure 5. Monomeric N terminus of EB3 tracks growing MT ends in cells. (A) Live images of CHO-K1 cells transfected with GFP-EB1-CΔAc alone or together with EB3-mRFP. Images were processed by applying Blur filter and Unsharp Mask in Photoshop. Bar, 5 µm. (B–G) Live images of control CHO-K1 cells (B–D) or EB1/EB3-depleted cells (E–G) expressing the indicated constructs. Cells expressing similar low levels of the fusion proteins were selected based on average fluorescence intensity. Bar, 5 µm. (H) Ratio of fluorescence intensities at the growing MT tips and in surrounding cytoplasm (after background subtraction), measured from live cell images obtained as described for panels B–G. 10 cells (45–200 MT tips) were measured for each construct; error bars represent SD. The differences between the indicated values were significantly different; statistical analysis was performed using nonparametric Mann-Whitney U test.
Figure 5.

Monomeric N terminus of EB3 tracks growing MT ends in cells. (A) Live images of CHO-K1 cells transfected with GFP-EB1-CΔAc alone or together with EB3-mRFP. Images were processed by applying Blur filter and Unsharp Mask in Photoshop. Bar, 5 µm. (B–G) Live images of control CHO-K1 cells (B–D) or EB1/EB3-depleted cells (E–G) expressing the indicated constructs. Cells expressing similar low levels of the fusion proteins were selected based on average fluorescence intensity. Bar, 5 µm. (H) Ratio of fluorescence intensities at the growing MT tips and in surrounding cytoplasm (after background subtraction), measured from live cell images obtained as described for panels B–G. 10 cells (45–200 MT tips) were measured for each construct; error bars represent SD. The differences between the indicated values were significantly different; statistical analysis was performed using nonparametric Mann-Whitney U test.

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