Figure 4. Expression of the dominant-negative EB1 mutant disrupts persistent MT growth in CHO-K1 cells. (A and B) Representative time-lapse images of YFP-CLIP-170 in cells expressing HA-EB1-NL (control) and HA-EB1-CΔAc (dominant negative) mutants. Images were acquired every 2 s. Tips of three YFP-CLIP-170 comets are indicated over time by an arrow, an arrowhead, and a black-and-white arrowhead. Projection analysis and trajectories of individual YFP-CLIP-170 comets are generated in the same way as in Fig. 2 A. Time in seconds is shown in the top right corner. Bar, 10 µm. (C and D) Histograms of the instantaneous MT growth rates and the lengths of YFP-CLIP-170 tracks in HA-EB1-NL and HA-EB1-CΔAc-expressing cells. (E) Distributions of the growing MT plus-ends along the cell radius in EB1-NL and EB1-CΔAc–expressing cells presented as in Fig. 2 E; error bars represent SD.
Figure 4.

Expression of the dominant-negative EB1 mutant disrupts persistent MT growth in CHO-K1 cells. (A and B) Representative time-lapse images of YFP-CLIP-170 in cells expressing HA-EB1-NL (control) and HA-EB1-CΔAc (dominant negative) mutants. Images were acquired every 2 s. Tips of three YFP-CLIP-170 comets are indicated over time by an arrow, an arrowhead, and a black-and-white arrowhead. Projection analysis and trajectories of individual YFP-CLIP-170 comets are generated in the same way as in Fig. 2 A. Time in seconds is shown in the top right corner. Bar, 10 µm. (C and D) Histograms of the instantaneous MT growth rates and the lengths of YFP-CLIP-170 tracks in HA-EB1-NL and HA-EB1-CΔAc-expressing cells. (E) Distributions of the growing MT plus-ends along the cell radius in EB1-NL and EB1-CΔAc–expressing cells presented as in Fig. 2 E; error bars represent SD.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal