Figure 1.
Figure 1. Effects of depletion of individual EBs on the distribution of other EB1 family members. (A–D) Immunofluorescent staining of the CHO-K1 cells for EB1, EB2, and EB3 after knocking down EB1 (A); EB2 (B); EB3 (C); or EB1 and EB3 simultaneously (D). EB-depleted cells are indicated by dotted lines. Bar, 10 µm. Enlarged insets from control (1) and EB-depleted cells (2) are shown in color; EB1 is red, EB2 is green, and EB3 is blue in the overlays. Bar, 5 µm. Averaged intensity distributions for each EB protein (±SEM) were obtained for 15–25 MT ends per condition; the values obtained in control cells from each experiment were used for normalization. Intensity distributions are shown for a control cell (a) and for cells depleted of EB1 (a′), EB2 (b), EB3 (c), or EB1 and EB3 simultaneously (d).

Effects of depletion of individual EBs on the distribution of other EB1 family members. (A–D) Immunofluorescent staining of the CHO-K1 cells for EB1, EB2, and EB3 after knocking down EB1 (A); EB2 (B); EB3 (C); or EB1 and EB3 simultaneously (D). EB-depleted cells are indicated by dotted lines. Bar, 10 µm. Enlarged insets from control (1) and EB-depleted cells (2) are shown in color; EB1 is red, EB2 is green, and EB3 is blue in the overlays. Bar, 5 µm. Averaged intensity distributions for each EB protein (±SEM) were obtained for 15–25 MT ends per condition; the values obtained in control cells from each experiment were used for normalization. Intensity distributions are shown for a control cell (a) and for cells depleted of EB1 (a′), EB2 (b), EB3 (c), or EB1 and EB3 simultaneously (d).

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