Effect of overexpressing Pld in shi mutants. TEM analysis of an ommatidium from shi¹ (A) Pld overexpression (B) and overexpression of Pld in shi¹ (C). Rhabdomeres (r) are marked. (D–F) Higher magnification views of the rhabdomere and cell body from a single photoreceptor showing tubulovesicular membranes in the cell body (V). (G) Quantitative analysis of rhabdomere biogenesis defects. (H) TEM analysis of photoreceptors from GMR-GAL4* UAS-RNAi^α-adaptin in an otherwise wild-type background. Individual rhabdomeres are smaller and ill formed. The cell bodies show the accumulation of membranous structures (m). (I) Overexpression of UAS-RNAi^α-adaptin and UAS-Pld using GMR-GAL4. The nucleus (n) and the pigment granules (p) normally found at the base of the rhabdomeres are marked. A representative example is shown with virtually no detectable rhabdomeres. The cell bodies of the photoreceptors from such animals continued to show accumulation of membrane transport-like intermediates. Bars: (A) 2 µm; (D and H) 1 µm.