Effects of Pld overexpression in photoreceptors with altered Arf1 activity. TEM analysis of photoreceptors. (A–C and E) Wild-type (A) overexpressing Pld (B), garz (C), and Pld and garz together (E). (G) Representation of the activity states of Arf1 and the enzymes that convert Arf1 between these states. Arf1-GTP, GTP-bound Arf1; Arf1-GDP, GDP-bound Arf1. (D and F) TEM analysis of photoreceptors overexpressing dArf1-GAP Pld (D) and dArf1-GAP (F). (H) Quantitative analysis of rhabdomere biogenesis defects. Genotypes are marked. The x axis shows bins for ommatidia with a given number of rhabdomeres developed. The y axis shows frequency of rhabdomeres falling within a given bin for each of the genotypes. At least 50 ommatidia were analyzed from three separate heads. r, rhabdomere; n, nuclei. Bar, 2 µm.