Lipidomic analysis of changes in PA and other phospholipids. Analysis of PA levels in photoreceptors by mass spectrometry. The acyl chain composition of each species predicted from its M r is shown on the x axis. The y axis shows the abundance of each species as a percent of total PA in that sample. (A–C) Elevation of 16:0/18:2 PA in cds1 relative to wild type (A), in flies overexpressing Pld but not K/R Pld or controls (B), and in flies overexpressing rdgA (C). The elevated species is marked in red on the x axis and is indicated by **. Levels of PC, DAG, and PI were measured from retinae of the following three genotypes: cds1 (D), Pld overexpression (E), and rdgA (F). Error bars indicate mean ± SD of the 34:2 species of each phospholipid.
