Figure 7. Exogenous expression of Ndc1 and Pom152 rescues the rtn1Δ yop1Δ Nup mislocalization phenotype. (A and B) The rtn1Δ yop1Δ GFP-nic96 (SWY3931; A) and the rtn1Δ yop1Δ (SWY3811; B) strains were transformed with plasmids expressing NDC1 (pRS315.NDC1; Chial et al., 1998), POM152 (pSW229), POM34 (pSW1517; Miao et al., 2006), or SNL1 (pSW575; Ho et al., 1998). Direct fluorescence microscopy (A) or indirect immunofluorescence microscopy with anti–Nup116-GLFG antibodies (B) was conducted to evaluate NPC clusters. DIC, differential interference contrast. Bars, 10 µm.
Figure 7.

Exogenous expression of Ndc1 and Pom152 rescues the rtn1Δ yop1Δ Nup mislocalization phenotype. (A and B) The rtn1Δ yop1Δ GFP-nic96 (SWY3931; A) and the rtn1Δ yop1Δ (SWY3811; B) strains were transformed with plasmids expressing NDC1 (pRS315.NDC1; Chial et al., 1998), POM152 (pSW229), POM34 (pSW1517; Miao et al., 2006), or SNL1 (pSW575; Ho et al., 1998). Direct fluorescence microscopy (A) or indirect immunofluorescence microscopy with anti–Nup116-GLFG antibodies (B) was conducted to evaluate NPC clusters. DIC, differential interference contrast. Bars, 10 µm.

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