Figure 6. Genetic interactions for the rtn1Δ yop1Δ mutant with mutants in genes encoding Nups, Poms, and ER/NE proteins. (A) Analysis of rtn1Δ yop1Δ nup/pom mutants. Haploid strains with the designated chromosomal deletions and wild-type RTN1, POM, or NUP URA3/CEN plasmid were tested for growth on 5-FOA media (SWY3811 + pRS316, SWY3876 + pSW3354, SWY3877 + pSW1079, SWY3878 + pSW1516, SWY4050 + pSW351, SWY4099 + pSW610, and SWY4106 + pSW3459). (B) Rescue of pom34Δ nup59Δ cells (SWY3139) by exogenous expression of RTN1-GFP or rtn1-K48I–GFP. The pom34Δ nup59Δ parental strain harboring a POM34/URA3 plasmid and LEU2 plasmids for POM34, RTN1-GFP, or rtn1-K48I–GFP were assayed for growth on 5-FOA media (23°C for 6 d). (C) Growth of 5-FOA–resistant pom34Δ nup59Δ isolates expressing POM34, rtn1-GFP, rtn1-K48I–GFP, RTN1, or rtn1-K48I was assessed after serial dilution on YPD.
Figure 6.

Genetic interactions for the rtn1Δ yop1Δ mutant with mutants in genes encoding Nups, Poms, and ER/NE proteins. (A) Analysis of rtn1Δ yop1Δ nup/pom mutants. Haploid strains with the designated chromosomal deletions and wild-type RTN1, POM, or NUP URA3/CEN plasmid were tested for growth on 5-FOA media (SWY3811 + pRS316, SWY3876 + pSW3354, SWY3877 + pSW1079, SWY3878 + pSW1516, SWY4050 + pSW351, SWY4099 + pSW610, and SWY4106 + pSW3459). (B) Rescue of pom34Δ nup59Δ cells (SWY3139) by exogenous expression of RTN1-GFP or rtn1-K48I–GFP. The pom34Δ nup59Δ parental strain harboring a POM34/URA3 plasmid and LEU2 plasmids for POM34, RTN1-GFP, or rtn1-K48I–GFP were assayed for growth on 5-FOA media (23°C for 6 d). (C) Growth of 5-FOA–resistant pom34Δ nup59Δ isolates expressing POM34, rtn1-GFP, rtn1-K48I–GFP, RTN1, or rtn1-K48I was assessed after serial dilution on YPD.

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