Figure 5. The rtn1Δ yop1Δ mutant has Nab2NLS-GFP nuclear import defects. Parental (BY4742) and rtn1Δ yop1Δ (SWY3811) cells with a plasmid expressing Nab2NLS-GFP (pNS167; Shulga et al., 2000) were grown to early log phase at 23°C. Import kinetics were determined after shifting energy-depleted cells with cytoplasmic Nab2NLS-GFP to glucose-containing media. (A) Localization of Nab2NLS-GFP by direct fluorescence microscopy at time points (minutes) after reinitiating import. (B) Kinetic data for three independent trials. Error bars represent standard deviation. Bar, 10 µm.
Figure 5.

The rtn1Δ yop1Δ mutant has Nab2NLS-GFP nuclear import defects. Parental (BY4742) and rtn1Δ yop1Δ (SWY3811) cells with a plasmid expressing Nab2NLS-GFP (pNS167; Shulga et al., 2000) were grown to early log phase at 23°C. Import kinetics were determined after shifting energy-depleted cells with cytoplasmic Nab2NLS-GFP to glucose-containing media. (A) Localization of Nab2NLS-GFP by direct fluorescence microscopy at time points (minutes) after reinitiating import. (B) Kinetic data for three independent trials. Error bars represent standard deviation. Bar, 10 µm.

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