Figure 4. NPCs are clustered and morphologically abnormal in rtn1Δ yop1Δ cells. (A–J) Parental cells (BY4742; A, C, and D) or rtn1Δ yop1Δ cells (SWY3811; B and E–J) were grown to early log phase at 23°C and processed for thin-section EM. Black and white arrows point to the NPC, black arrowheads point to NPC-like structures at the ONM, white arrowheads point to NPC-like structures at the INM, and the asterisks denote other aberrant NPC-like structures at the NE. N, nucleus; C, cytoplasm; vac, vacuole. Bars, 0.5 µm.
Figure 4.

NPCs are clustered and morphologically abnormal in rtn1Δ yop1Δ cells. (A–J) Parental cells (BY4742; A, C, and D) or rtn1Δ yop1Δ cells (SWY3811; B and E–J) were grown to early log phase at 23°C and processed for thin-section EM. Black and white arrows point to the NPC, black arrowheads point to NPC-like structures at the ONM, white arrowheads point to NPC-like structures at the INM, and the asterisks denote other aberrant NPC-like structures at the NE. N, nucleus; C, cytoplasm; vac, vacuole. Bars, 0.5 µm.

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