Figure 3. Nups mislocalize in the rtn1Δ yop1Δ mutant. (A) Parental cells (SWY1695 or SWY809) or rtn1Δ yop1Δ cells expressing either GFP-Nic96 or Nup49-GFP (SWY3931 or SWY3880) were grown to early log phase at 23°C and were visualized by direct fluorescence microscopy. (B) Parental cells (BY4742) or rtn1Δ yop1Δ cells (SWY3811) were grown to early log phase at 23°C and processed for indirect immunofluorescence microscopy with anti-Nup116C or anti–Nup116-GLFG antibodies. Nuclei were detected with the DNA dye DAPI. DIC, differential interference contrast. Bars, 10 µm.
Figure 3.

Nups mislocalize in the rtn1Δ yop1Δ mutant. (A) Parental cells (SWY1695 or SWY809) or rtn1Δ yop1Δ cells expressing either GFP-Nic96 or Nup49-GFP (SWY3931 or SWY3880) were grown to early log phase at 23°C and were visualized by direct fluorescence microscopy. (B) Parental cells (BY4742) or rtn1Δ yop1Δ cells (SWY3811) were grown to early log phase at 23°C and processed for indirect immunofluorescence microscopy with anti-Nup116C or anti–Nup116-GLFG antibodies. Nuclei were detected with the DNA dye DAPI. DIC, differential interference contrast. Bars, 10 µm.

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