Figure 2. Rtn1-GFP and Yop1-GFP are associated with NPC clusters in the nup133Δ mutant. (A and B) Indirect immunofluorescence microscopy was conducted with parental rtn1-GFP cells (A, top), nup133Δ rtn1-GFP cells (SWY4007; A), parental yop1-GFP cells (B, top), or nup133Δ yop1-GFP cells (SWY4045; B) grown to early log phase at 23°C using rabbit anti-GFP and mouse anti–FG Nup mAb414 antibodies. Direct laser-scanning confocal microscopy was used with single-channel fluorescence for the GFP and mAb414 staining shown from three representative fields. Merged images are shown (right). (C) Live cell direct fluorescence microscopy was conducted with rtn1-GFP nic96-mCherry (SWY4125) and rtn1-GFP nic96-mCherry nup133Δ cells (SWY2117) grown to early log phase at 23°C. DIC, differential interference contrast. Bars: (A and B) 2.5 µm; (C) 10 µm.
Figure 2.

Rtn1-GFP and Yop1-GFP are associated with NPC clusters in the nup133Δ mutant. (A and B) Indirect immunofluorescence microscopy was conducted with parental rtn1-GFP cells (A, top), nup133Δ rtn1-GFP cells (SWY4007; A), parental yop1-GFP cells (B, top), or nup133Δ yop1-GFP cells (SWY4045; B) grown to early log phase at 23°C using rabbit anti-GFP and mouse anti–FG Nup mAb414 antibodies. Direct laser-scanning confocal microscopy was used with single-channel fluorescence for the GFP and mAb414 staining shown from three representative fields. Merged images are shown (right). (C) Live cell direct fluorescence microscopy was conducted with rtn1-GFP nic96-mCherry (SWY4125) and rtn1-GFP nic96-mCherry nup133Δ cells (SWY2117) grown to early log phase at 23°C. DIC, differential interference contrast. Bars: (A and B) 2.5 µm; (C) 10 µm.

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