TOR pathway–driven transcriptional changes are conserved in eukaryotes. (A) Northern analysis of the hsp27, PSPH, REDD1, REDD2, and GAPDH genes in primary Tsc2 (−/−) or wild-type littermate control (+/+) MEFs. (B) Schematic representation of the FRE in the REDD1 promoter. (C) Luciferase assay (n = 4) measuring transcriptional activity of either wild-type (WT) or FRE mutant (mtFRE) REDD1 promoters in primary MEFs expressing either vector alone (Vector) or FOXO GOF (FOXO). (D) Transcriptional activity of the REDD1 wild type or FRE mutant promoter in wild-type (wt) or Tsc2 MEFs (n = 6). Error bars represent standard deviation.