FOXO is elevated in Tsc1 tissue and inhibits growth of Tsc1 organs. (A and B) Confocal microscope images of a Tsc1 mosaic wing imaginal disc. FOXO expression (red in A and in the merged image shown in B) was elevated in Tsc1 clones when compared with wild-type tissue, which expresses GFP (green in B). (C) QPCR analysis of 4e-bp, aay, and scy mRNAs normalized to a tubulin (tub) control mRNA in eye-antennal imaginal disc tissue of several genotypes: wild-type (wt), Tsc1, or Tsc1-FOXO (T-F). (D–G) Scanning electron micrographs of adult eyes of genotypes: wild type(D and D′), Tsc1 (E and E′), Tsc1-tgo (F and F′), and Tsc1-FOXO (G and G'). Data are represented as a mean expression level derived from between three and nine replicate experiments performed on pooled biological samples collected from at least 20 animals of each genotype. However, n was still equal to 1. Therefore, data were averaged to provide mean expression data but did not allow measurement of error values such as standard deviation or standard error of the mean. Bars: (A and B) 50 μM; (D–G) 100 μM.