Figure 3. Invadopodia assembly and disassembly rates are reduced in calpain 2–deficient MTLn3 cells. (A) GFP-actin or (B) GFP-cortactin were transiently transfected into MTLn3 control and calpain 2 siRNA lines. Cells were plated on FN-coated glass-bottomed dishes and analyzed by time-lapse fluorescence microscopy. The time-lapse montage of calpain-deficient cells expressing GFP-actin (A) or GFP-cortactin (B) demonstrates that GFP-actin or GFP-cortactin persist at invadopodia for longer durations in calpain-deficient MTLn3 cells. Arrows indicate representative invadopodia. Duration measurements and assembly and disassembly rates are shown. Data show means ± SEM from four independent experiments. Asterisks indicate statistical significance compared with control based on a t test (*, P < 0.03; **, P < 0.05). See Videos 1–4 (available). Bars, 10 μm.
Figure 3.

Invadopodia assembly and disassembly rates are reduced in calpain 2–deficient MTLn3 cells. (A) GFP-actin or (B) GFP-cortactin were transiently transfected into MTLn3 control and calpain 2 siRNA lines. Cells were plated on FN-coated glass-bottomed dishes and analyzed by time-lapse fluorescence microscopy. The time-lapse montage of calpain-deficient cells expressing GFP-actin (A) or GFP-cortactin (B) demonstrates that GFP-actin or GFP-cortactin persist at invadopodia for longer durations in calpain-deficient MTLn3 cells. Arrows indicate representative invadopodia. Duration measurements and assembly and disassembly rates are shown. Data show means ± SEM from four independent experiments. Asterisks indicate statistical significance compared with control based on a t test (*, P < 0.03; **, P < 0.05). See Videos 1–4 . Bars, 10 μm.

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