Loss of miR-8 miRNAs alters apical membrane trafficking. (A–D) FITC–Con A (green) and MitoTracker red (red) staining was performed to examine the apical membranes of H⁺ pump–rich ionocytes. Con A staining in UIC, AB^MO1-injected embryos, nherf1^MO-injected embryos, and AB^MO1- and nherf1^MO-coinjected embryos. Live embryos were imaged with each panel showing a view of cells on the surface of the yolk sac and the inset showing a side view of the z stack with apical basal localization of Con A staining. (E and F) Mean number of ungrouped (E) or internalized (F) foci of Con A staining from embryos in A–D. Error bars represent SEM. Statistical significance is indicated by the asterisks and was determined by ANOVA at α ≤ 0.05 (n = 14 for UIC, n = 11 for AB^MO1, n = 13 for nherf1^MO, and n = 10 for nherf1^MO+ AB^MO1). Data were gathered from three independent experiments.