ILK siRNA results in aberrant mitotic spindles, misaligned chromosomes, and the prevention of progress through mitosis. (A) Western blot of HeLa cell extracts after ILK siRNA treatment. The amount of ILK remaining after knockdown is shown as a percentage of ILK in control siRNA and is adjusted according to β-actin levels. (B) HeLa cells treated with control or ILK siRNA show similar aberrant spindle and misaligned chromosome phenotypes to the QLT-0267–treated cells. Bars, ∼10 μm. (C–E) Histograms showing the percentage of mitotic cells with aberrant spindles (C), with misaligned chromosomes (D), and in the various stages of cell division (E) in ILK and control siRNA-treated cells. Data are mean ± SD from three independent experiments in which >50 mitotic cells were analyzed.