Figure 1. ILK interacts with tubulin,... | Rockefeller University Press

Figure 1.

$Figure 1. ILK interacts with tubulin, RUVBL1, and ch-TOG and localizes to centrosomes. (A) FLAG-ILK was immunoprecipitated from the cytoskeleton of HEK293 cells, and the presence of α- and β-tubulin, ch-TOG, and RUVBL1 was determined by Western blot. (B) Detergent-soluble fractions (S1 and S2), isolation buffer–soluble fraction (S3), and purified mitotic spindles (P) from HeLa cells Western blotted for indicated proteins are shown. (C–H) HeLa cells costained for ILK and indicated proteins as well as DNA. Insets are close-ups of small squares (C) or of centrosomes (F). Arrowheads, centrosomes; arrow, focal adhesions. Bars, ∼10 μm.$

ILK interacts with tubulin, RUVBL1, and ch-TOG and localizes to centrosomes. (A) FLAG-ILK was immunoprecipitated from the cytoskeleton of HEK293 cells, and the presence of α- and β-tubulin, ch-TOG, and RUVBL1 was determined by Western blot. (B) Detergent-soluble fractions (S1 and S2), isolation buffer–soluble fraction (S3), and purified mitotic spindles (P) from HeLa cells Western blotted for indicated proteins are shown. (C–H) HeLa cells costained for ILK and indicated proteins as well as DNA. Insets are close-ups of small squares (C) or of centrosomes (F). Arrowheads, centrosomes; arrow, focal adhesions. Bars, ∼10 μm.