Figure 1.
Figure 1. Poly(A)+ RNA export is inhibited in sem1Δ but is not impaired in mutants of the RP. (A) The depicted yeast strains were grown in YPD at 30°C; the localization of poly(A)+ RNA in the strains was assessed by in situ hybridization using Cy3-oligo (dT)30, and DNA was stained with DAPI. The mex67-5 strain was grown at 25°C and subsequently shifted to 37°C for 1 h. (B) The cim3-1 and cim5-1 were grown to logarithmic phase at 25°C and subsequently shifted to 37°C for 4 h. Other details were as in A.

Poly(A)+ RNA export is inhibited in sem1Δ but is not impaired in mutants of the RP. (A) The depicted yeast strains were grown in YPD at 30°C; the localization of poly(A)+ RNA in the strains was assessed by in situ hybridization using Cy3-oligo (dT)30, and DNA was stained with DAPI. The mex67-5 strain was grown at 25°C and subsequently shifted to 37°C for 1 h. (B) The cim3-1 and cim5-1 were grown to logarithmic phase at 25°C and subsequently shifted to 37°C for 4 h. Other details were as in A.

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