Increased c-Jun binding to AP-1 site of human KGF, GMCSF, and IL-6 gene promoter in F_sIL-1ra. ChIP of AP-1–binding site of human KGF (top), GMCSF (middle), and IL-6 (bottom) genes using phospho–c-Jun antibodies. The gene sequence spanning the AP-1 site and a random control sequence were analyzed by PCR in the immunoprecipitated chromatin of F_CTRL and F_sIL-1ra fibroblasts extracted from K/F_CTRL and K/F_sIL-1ra OTC, respectively. Preimmune serum was used as a control. qPCR was performed on immunoprecipitates of phospho–c-Jun antibodies and normalized to input (chromatin before immunoprecipitation). Results are represented in fold change as compared with F_CTRL extracted from K/F_CTRL OTC. M, 100 bp DNA ladder.