Figure 3.
Figure 3. ICAP-1 loss induces a faster recruitment of talin into FAs.Icap-1+/+ and Icap-1−/− MEF cells were seeded in LabTekII chambers coated with either 20 μg/ml FN (A) or 5 μg/ml VN (B) and were allowed to spread for 12 h. After transfection, both cell types expressing either EGFP-talin or EGFP-vinculin were subjected to FRAP experiments. Photobleaching was performed on manually delimited individual FAs localized at the edge of cells. EGFP fluorescence recovery was measured at 1-min intervals over a 15–20-min period, and the characteristic recovery time (τ) of each FA protein was estimated. At least 20 FAs were recorded for both cell types. Error bars indicate SD. The shorter recovery time, τ, of EGFP-talin in Icap-1−/− cells indicates a higher mobility of this protein (e.g., its faster recruitment into FA). *, P ≤ 0.01.

ICAP-1 loss induces a faster recruitment of talin into FAs. Icap-1+/+ and Icap-1−/− MEF cells were seeded in LabTekII chambers coated with either 20 μg/ml FN (A) or 5 μg/ml VN (B) and were allowed to spread for 12 h. After transfection, both cell types expressing either EGFP-talin or EGFP-vinculin were subjected to FRAP experiments. Photobleaching was performed on manually delimited individual FAs localized at the edge of cells. EGFP fluorescence recovery was measured at 1-min intervals over a 15–20-min period, and the characteristic recovery time (τ) of each FA protein was estimated. At least 20 FAs were recorded for both cell types. Error bars indicate SD. The shorter recovery time, τ, of EGFP-talin in Icap-1−/− cells indicates a higher mobility of this protein (e.g., its faster recruitment into FA). *, P ≤ 0.01.

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