PH-Akt-GFP translocation to leading edge and phagocytic cup. (a) The localization of PH-Akt-GFP (green) and DiIC₁₆(3) (red) in a polarized neutrophilic HL60 cell shows the specificity of PH-Akt-GFP fluorescence to the membrane at the front of the cell. (b) The distribution of PH-Akt-GFP (green) and DiIC₁₆(3) (red) in a neutrophilic HL60 cell during phagocytosis is shown, together with a ratio image of the two signals showing the specificity of binding of PH-Akt-GFP to the phagocytic cup. Bar, 5 µm (data taken from Dewitt et al., 2006).