Wld^S localizes to the nucleus in Drosophila S2 cells and associates with TER94, and TER94 is essential for Wld^S-like levels of axon protection. (A and B) S2 cells were transfected with Wld^S-myc and stained with α-Myc antibodies, and α-Myc immunoreactivity localized to the nucleus. (C) Immunoprecipitation (IP) from Wld^S-Myc–transfected cell lysates with α-Myc antibodies led to the purification of a 97-kD isoform of Ter94. HC, heavy chain from α-Myc IgG; WB, Western blot. (D) UAS-Ter94^RNAi was driven in OR22a⁺ ORNs in the presence or absence of Wld^S. n ≥ 10 for all genotypes; *, P < 0.05; **, P < 0.01; ***, P < 0.001. Error bars represent SEM. Bar, 13.56 µm.