Figure 2. WldS variant proteins are stably expressed in Drosophila neurons. Three independent UAS-driven transgene insertion lines for each WldS variant molecule were crossed to elav-Gal4, and protein extracts from heads were assayed for expression using α-Nmnat1 antibodies. Multiple lines for each construct were found to express detectable levels of protein. The expression of each of these molecules in ORNs with OR22a-Gal4 did not lead to expression of proteins at levels detectable by Western blot analysis (not depicted). Molecular masses are shown in kilodaltons. MW, molecular weight.
Figure 2.

WldS variant proteins are stably expressed in Drosophila neurons. Three independent UAS-driven transgene insertion lines for each WldS variant molecule were crossed to elav-Gal4, and protein extracts from heads were assayed for expression using α-Nmnat1 antibodies. Multiple lines for each construct were found to express detectable levels of protein. The expression of each of these molecules in ORNs with OR22a-Gal4 did not lead to expression of proteins at levels detectable by Western blot analysis (not depicted). Molecular masses are shown in kilodaltons. MW, molecular weight.

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