Type IV 5-ptase mediates decrease of PIP2 and induces endogenous ERM protein dephosphorylation in Jurkat cells. (A) Immunofluorescence analysis of ERM protein phosphorylation in cells transfected with PH-GFP construct, the membrane-targeted FRB-CFP, and the mRFP-FKBP domain construct (with or without type IV 5-ptase domain as indicated). (B) Quantitative analysis of pERM (n = 10 for each condition). Error bars indicate SEM. Rapa, rapamycin; CT, control.